This Application Note describes the applicability of the P200 ScreenTape system for: • fully automated electrophoretic analysis of IEX fractions • screening of protein fractions from His-tag affi nity purifi cations • analysis of column fractions from MBP-tag purifi cations • monitoring and optimization of the effi ciency of the GST-tag cleavage reaction
The document details how the Agilent 2200 TapeStation instrument combined with the P200 ScreenTape consumable provides an automated electrophoresis analysis system. It is designed for proteins ranging from 10 to 200 kDa and handles a broad range of concentrations and sample buffers, facilitating the optimization and control of protein purification workflows.
| Protein size range | 10 to 200 kDa |
|---|---|
| Analysis time per sample | less than one minute |
The system is applicable to IEX fractions, His-tag affinity purification fractions, MBP-tag purification column fractions, and GST-tag cleavage reactions.
The system analyzes 16 protein samples in less than one minute per sample.
The Agilent 2200 TapeStation instrument analyzes proteins ranging from 10 to 200 kDa.
A GST-tagged protein at 0.4 µg/µL was cleaved overnight at 8 °C.