This Application Note describes a method for the simultaneous quantitation of five artificial sweeteners, acesulfame K, aspartame, alitame, neotame, and saccharin in diet colas using an Agilent 1260 Infinity LC system. With the 60-mm Max-Light flow cell, all sweeteners could be detected at 210 nm. The linear dynamic ranges were determined after validating the robustness of seven method parameters. All the analytes showed good linearity up to 50 µg/mL with the R2 values being > 0.9999. Acesulfame K, aspartame, and alitame had LOD values of 0.1 µg/mL and LOQ values of 0.2 µg/mL, while the LOD of saccharin was found to be 0.05 µg/mL and LOQ to be 0.1 µg/mL. For neotame, both the LOD and LOQ values were 0.5 µg/mL. Two diet cola samples were analyzed by this method and excellent recoveries were obtained for the spiked samples.
The document details a method for analyzing artificial sweeteners (acesulfame K, aspartame, alitame, neotame, and saccharin) in diet cola beverages. The analysis utilizes an Agilent 1260 Infinity LC system equipped with a Diode Array Detector featuring a 60-mm Max-Light flow cell.
The method quantifies acesulfame K, aspartame, alitame, neotame, and saccharin.
All analytes showed good linearity up to 50 pg/mL with R² values > 0.9999.
The LOD value for saccharin was found to be 0.05 pg/mL.