Solid-phase extraction (SPE) coupled with high-performance liquid chromatography (HPLC) was optimized for extraction and quantification of two fungicides (carbendazim and thiabendazole) in apple juice. Results indicate that SPE using Agilent Bond Elut SCX (60 mg, 3 mL) and HPLC using an Agilent ZORBAX Eclipse Plus C18 column (4.6 mm × 100 mm, 3.5 µm) is an excellent combination for extraction and analysis of these compounds. Recoveries ranged from 92.1 to 99.4 percent with RSDs below 5 percent and limits of detection of 4 µg/kg.
This document details an optimized method for extracting and quantifying carbendazim and thiabendazole fungicides from apple juice. The procedure utilizes Agilent Bond Elut SCX cartridges coupled with High-Performance Liquid Chromatography (HPLC) using a ZORBAX Eclipse Plus C18 column.
The study validates the method based on SN/T 1753-2006, reporting recoveries between 92.1% and 99.4%, relative standard deviations below 5%, and limits of detection at 4 µg/kg.
The limit of detection for both fungicides is 4 pg/kg.
Recoveries for carbendazim ranged from 95.9% to 99.4%, while recoveries for thiabendazole ranged from 92.1% to 99.0%.
The sample should be loaded at a speed of about 1 mL/min.