A method for simultaneous determination of 11 β2-agonist residues of clebuterol, salbutamol, ractopamine, terbutaline, salmeterol, propranolol, tulobuterol, cimaterol, mabuterol, mapenterol, and zilpaterol in pork has been developed and validated. The analytes are extracted by liquid-liquid extraction (LLE) and solid-phase extraction (SPE) and quantified by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) operating in positive ion multiple reac- tion monitoring (MRM) mode. The method provides a pg/g level of limit of detection (LOD) for all β2-agonists in pork. The dynamic calibration ranges for these compounds are obtained from 0.25 to 5 ng/g. The overall recoveries range from 82% to 105% with RSD values between 1.6% and 8.4%.