Industrial

This Application Note describes how the Agilent 2100 bioanalyzer and the cell fluorescence LabChip® kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a reporter molecule by on-chip staining. Transfection of CHO-K1 cells with an expression vector encoding GFP was performed and cells were stained on-chip with the live-dye CBNF (carboxynaphthofluorescein). The histogram quality and the percent transfected cells determined are in good agreement with data obtained using a conventional flow cytometer. Detailed protocols and reagent recommendations for analyzing transfection reactions are provided. Speed of the on-chip staining procedure, high reproducibility of the chip results, low cell consumption and ease-of-use are advantages the Agilent 2100 bioanalyzer offers for monitoring transfection efficiency using GFP.

This Application Note investigates the various techniques for RNA quantitation, including a comparison of the Agilent 2100 bioanalyzer with UV spectrometry and the Ribogreen® quantitation system to access the linear dynamic range, reproducibility, and the effects of contaminants for each quantitation method.

This article describes the analysis of nucleotides using an Agilent Polaris C18-A column. The HPLC separation of ten nucleotides was achieved in 24 minutes. The column has a particle size of 5 μm and is suitable for pharmaceutical applications.

Introduces the separation of four metanephrines in urine with an Agilent Polaris C18-A column

Agilent RNA 6000 Pico LabChip® kit can be used to control the quality of RNA in laser microdissected tissue samples

A robust method for detection of THC and its metabolites in blood has been developed using SPE extraction and GC/MS/MS with backflushing. The dynamic range of quantification was 0.1 to 50 ng/mL for THC and 11-OH-THC, and 1 to 100 ng/mL for THCA, with a run time of 6 minutes and a cycle time of 8 minutes.

This Application Note describes a method for the fast analysis of chemotherapy drugs using Agilent Polaris and Agilent Pursuit columns. The method allows for the differentiation of cancer drugs in less than three minutes and can be used to assess the quality of drug preparations. The development of this method is a major step in improving the quality of drug delivery for cancer patients.

This document describes the method of using Agilent ResiPore columns and gel permeation chromatography to analyze phenolic resins

This document introduces the application of Agilent OligoPore and gel permeation chromatography to analyze silane oligomers, which are generated by the controlled hydrolysis of silanes under acidic conditions, and the silane oligomers are then analyzed by Agilent OligoPore columns.

This document describes the use of Agilent OligoPore and gel permeation chromatography for fingerprinting epoxy resins. The distribution of oligomers in the resin determines its physical properties, such as curing time and mechanical strength. The analysis of oligomer distribution using gel permeation chromatography allows for the fingerprinting of different grades of resin, which is crucial for quality and process control. The authors demonstrate the analysis using two commercial epoxy resins, Epikote 828 and Epikote 1001. Epikote 828 is dominated by a single oligomer peak, while Epikote 1001 contains multiple oligomers with increasing molecular weight. This method is of significant importance for resin quality and process control.

This application note describes the analysis of a sample of an ASA/ABS-blended material from a car door panel by gel permeation chromatography (GPC), using Agilent PLgel 5 µm MIXED-C columns.

This document describes a dried blood spot technology for drug analysis, including methods for collecting, storing, and analyzing blood samples.

Identifi cation and Quantitation of Pesticides in Chamomile and Ginger Extracts Using an Agilent 6460 Triple Quadrupole LC/MS system with Triggered MRM

This document describes the USP analysis of sorbitol on an Agilent Hi-Plex Pb column. Sorbitol is a sugar alcohol that is metabolized slowly by the body and is used as a sugar substitute in food and in tobacco products. It is also a common ingredient in toothpaste. Additionally, sorbitol is widely used in the pharmaceutical industry as both an excipient and an active ingredient.