Agilent Manuals

This article describes the effective analysis of low chromophore polymer additives using HPLC with ELSD. ELSD is a detection technique that is suitable for compounds with weak or no UV chromophores. The article mentions that Varian's evaporative light scattering detector is highly sensitive to a wide range of compounds and is ideal for this application. Analysis of polymer additives has been limited by the suitability of the detection method, as many additives lack a UV chromophore and are not suitable for refractive index detection. Therefore, there is a need for accurate, reliable, and robust analytical methods. The article also discusses the importance of polymer additives in polymers and their impact on polymer characteristics.

This application note describes a method for the high-sensitivity analysis of betaine in Chinese lycium using high-performance liquid chromatography (HPLC) with an electrochemical detection (ELSD). This method has high sensitivity and is suitable for the determination of betaine in plasma and other biological samples.

This application note describes a rapid and robust method for profiling carbohydrates in fruit juices using HPLC with ELSD.

This application note describes the LC-MS/MS method for determining malachite green (MG) and crystal violet (CV) using Pursuit™ XRs.

This application note describes how to remove salts from samples using StratoSpheres™ PL-MIXED MP cartridges to protect HPLC columns.

This application note describes the analysis of 45 neutral, basic, and acidic veterinary drugs in different meat matrices down to the MRLs (0.01 ppm) of the Japanese Positive List.

This application note provides a method for enhancing the detection sensitivity of tobramycin using pre-column derivatization with orthophthalaldehyde (OPA). The method can be used to analyze poorly detectable polar compounds, such as tobramycin.

This document describes a method for sample cleanup of tobramycin in serum using pre-SPE derivatization with orthophthalaldehyde (OPA) to enhance selectivity. The method improves the cleanup of tobramycin from a biological matrix by pre-SPE derivatization with OPA and enhances detection sensitivity through fluorescence detection of the derivative formed.

This document describes a method for the identification and quantification of pesticides and herbicides in water using liquid chromatography (LC) and diode array detector (DAD). The method was developed not only for the separation and detection of 17 common herbicides but also 19 additional herbicides and pesticides of possible interest. The methodology consists of: • Sample preparation by Solid Phase Extraction using Empore Disk technology • Separation by HPLC • Peak identification by Spectral Library Search using a Diode Array Detector

This article describes the method of quantitation of partially resolved peaks using multicomponent analysis (MCA) in high-performance liquid chromatography (HPLC) with diode array detection. MCA allows accurate quantitation and identification of unknown components even under partial or zero resolution conditions. It also provides a more thorough assessment of peak homogeneity compared to other purity assessment techniques. The method enables quantitation of small peaks fused to a major peak, even when they are not visually detected or integrated separately, without the need to select different optimum wavelengths to distinguish the compounds.

This application note describes the use of a fully automated on-line LC-GC system for the clean-up and determination of PAH in urban air particulate extracts. The system consists of a high-performance liquid chromatograph (LC) coupled on-line to a gas chromatograph (GC).

This application note describes a method for rapid separation and quantitation of a mixture of 10 non-steroidal anti-inflammatory drugs (NSAIDs) using fast high-performance liquid chromatography (HPLC) and diode array detector (DAD). Separations are performed in less than 5 minutes using a 3-cm long column. To enhance resolution, two wavelengths, 254 and 360 nm, are used. Quantitation is performed by integrating the peak areas of the absorbance peaks at each wavelength. This method can be used to rapidly identify and quantify the NSAIDs present in a mixture.

The document introduces the application of Star 9080 Amperometric Electrochemical Detector . The detector is used to analyze the standards of 30 nmol/L noradrenaline (1), adrenaline (2) and dopamine (3).

Application note: Analysis of amino acid neurotransmitters with Star 9080 EC detector