The use of a highly inert capillary column can facilitate the quantitation of low levels of very active compounds. The use of the highly inert HP-5MSi column in conjunction with the Agilent 6890/5973 inert gas chromatograph/mass spectrometer (GC/MS) system allows the measurement of pesticides and environmental contaminants at low levels while maintaining response linearity over a wide concentration range.
This document evaluates the performance of the new HP-5MSi (MS Inert) capillary column for the analysis of active compounds, specifically pesticides and environmental contaminants. The column features low bleed and is specially deactivated to minimize peak tailing caused by interactions with active sites in the sample flow path.
The evaluation includes testing with a specialized Test Mix #1 and linearity studies on various pesticides and semivolatile phenols and amines. Results demonstrate that the HP-5MSi column provides superior peak shapes and linear calibration curves compared to standard columns, allowing for the quantitation of compounds at low nanogram levels.
| Column Dimensions | 30 m x 0.25 mm x 0.25 µm |
|---|---|
| Carrier Gas (Test Mix #1) | He, 1 mL/min |
| Injector Temperature (Test Mix #1) | 250 °C |
| Oven Temperature Range (Test Mix #1) | 70 °C to 280 °C |
| Detector Temperature (MSD) | 280 °C |
The document tested terbofos, malathion, endrin aldehyde, DDT, 2,4-dinitrophenol, pentachlorophenol, and n-nitrosodipropylamine.
It exhibits much improved peak shapes and superior linearity with lower deviation in response factors compared to standard 5ms columns.