This article presents a method for quantitatively analyzing opiates in urine using liquid chromatography/mass selective detector time-of-flight (LC/MSD TOF). The method utilizes accurate mass measurement to enhance selectivity.
This document presents an application demonstrating the quantitative analysis of opiates in urine samples using Agilent liquid chromatography/mass selective detector time-of-flight (LC/MSD TOF). The study highlights the advantages of accurate mass measurement for enhancing selectivity and reducing chemical noise.
| Instrument | Agilent 1100 Series LC/MSD TOF |
|---|---|
| Column | ZORBAX XDB-C18, 2.1 mm x 50 mm, 3.5 pm |
| Mobile Phase A | Acetonitrile with 0.1 % formic acid |
| Mobile Phase B | Water with 0.1 % formic acid |
| Flow rate | 0.35 mL/min |
| Detection limit | 2 pg on-column |
The instrumental detection limit is 2 pg on-column with a signal/noise ratio of 5:1.
The document demonstrates both direct injection of urine and solid phase extraction (SPE).