This document describes a comprehensive method for profiling free and conjugated estrogens using capillary electrophoresis-time of flight-mass spectrometry. The method is sensitive and robust.
This document details a methodology for the comprehensive profiling of free and conjugated estrogens in complex biological fluids, such as urine. The method addresses the challenges of wide dynamic range and chemical diversity in endogenous and exogenous estrogen metabolites.
| Background Electrolyte | 50 mM ammonium bicarbonate pH 9.5 |
|---|---|
| Injection Pressure | 50 mbar |
| Injection Time | 5 seconds |
| Applied Voltage | 20 kV |
| Sheath Liquid Flow Rate | 14 pL/min |
| Mass Range | m/z 50-1100 |
| Mass Accuracy | <2 ppm |
The optimum condition is a capillary voltage of -4.0 kV.
The sheath liquid is 5 mM ammonium bicarbonate in 80:20 MeOH:H2O.