Agilent Manuals

This document introduces the method of using ZORBAX 300SB analytical column for protein separation. By optimizing the method, a quick and easy scale-up from analytical to semi-preparative HPLC can be achieved.

The document introduces the advantages of ZORBAX Poroshell packings in the HPLC separation of large molecules such as proteins. This surface porous particle can make the separation faster. At high pH, peptides and small proteins can also be separated by using Extend-C18-bonded Poroshell particles. The high pH may be desirable for improving selectivity, solubility, ease of downstream processing, or to change signal-to-noise and charge-state in LC/MS. By extending the gradient time, it is possible to achieve even higher resolution in typical run times of 10 to 20 min.

This document describes the use of mass spectrometry to identify unknown compounds in the pharmaceutical industry. By using a time-of-flight mass spectrometer with accurate mass capabilities, it is possible to determine the structure and composition of unknown compounds more accurately. This is important for the identification of unknown compounds during synthesis and manufacturing processes in the pharmaceutical industry.

This Application Note describes how to use the Agilent 2100 bioanalyzer to detect mutations in the K-ras and P16 genes.

The document introduces the use of Gel-Filtration standards and the performance testing of ZORBAX GF-250 columns. The ZORBAX GF-250 column can effectively separate the components of the Bio-Rad® gel-filtration standard between thyroglobulin (670 KDa) and Vitamin B12 (1.35 KDa). The document also discusses the application and results of using gel-filtration test mixtures to test ZORBAX GF-250 columns.

Differential proteome analysis: Two-dimensional nano LC/MS of E. coli proteome grown on different carbon sources

Glycoprotein sizing on the Agilent 2100 bioanalyzer

This document describes the method of removing salts from protein samples prior to analysis on the Agilent 2100 bioanalyzer. This can improve the sensitivity and consistency of the analysis.

ChemStation is a chemical analysis software developed by Agilent company, including basic software and data management module ChemStore. This software can generate reports, manage data, and perform second analysis on chemical analysis data.

This document describes the nature and location of all electronic data in a ChemStation Plus networked data system, including the add-on modules ChemStore as central database and Security Pack for support of compliance requirements. The document will help system and laboratory managers to understand how electronic results are managed by a ChemStation Plus system and how all related records are linked to a final result. The document will also help system administrators to understand the format of all ChemStation Plus electronic data, the data location and data organization in the database, and the operating system perspective. The ultimate goal of this document is to give every system user an outline of the ChemStation Plus structure, which will allow them to prove data integrity of their results and of the entire system during an audit or investigation. This document should be stored and managed carefully as the information contained herein could be misused to modify or manipulate ChemStation Plus electronic records.

A purification strategy for a medicinal chemistry group at Boehringer Ingelheim in Vienna, Austria, using the Agilent 1100 Series purification system was developed. The goal was to develop a simple purification process, which could be easily used by all chemists. Further challenges were to develop a set of pre-defined methods to purify not only simple samples but also problematic samples, such as insoluble samples, polar samples and samples that show poor ionization.

Agilent 1100 Series high throughput system introduction

This application note describes a method of using ICP-MS to measure surface metal contamination on silicon wafers by combining VPD with ICP-MS.

This Application Note demonstrates mass-based purification of a series of synthetic peptides with the Agilent 1100 Series purification system. The modular set-up of the system, a reliable fully-automated delay volume calibration and a comprehensive software package provide a versatile platform for purification tasks up to 100 mL/min. Crude peptides were successfully purified by reverse phase HPLC from less than 1 kDa to more than 10 kDa, covering a pI range from 4 to 13.

This article introduces the method of optimizing determination of C1-C6 impurities in propylene and ethylene using HP-PLOT/Al2O3 columns. Compared with other commercially available alumina PLOT columns, HP-PLOT columns have better selectivity, increased capacity and complete baseline separation. HP-PLOT column analyses were done using the 5890 Series II gas chromatograph with electronic pressure control and a flame ionization detector.