Agilent Manuals

This application is a tool to aid RNA data interpretation and identify features from total RNA electropherograms that reveal information about RNA sample quality.

This Application Note describes the general use of cell fluorescence assays on the Agilent 2100 bioanalyzer. The cell assay extension together with the cell fluorescence LabChip® kit allows both the analysis of pre-stained cells and the analysis of cells by on-chip staining. Cell assays on the 2100 bioanalyzer are based on simple flow cytometric analysis. The technical principles, as well as the typical workflow in sample and chip preparation are described here. A typical dye optimization experiment was performed and the appropriate dye concentration for a specific cell line determined. The cell fluorescence software is easy-to-use and allows different forms of data evaluation and result presentation. Using an example, data evaluation is explained in detail. Specific advantages of cell fluorescence analysis with the Agilent 2100 bioanalyzer are low cell and reagent consumption, ease-of-use, automated data acquisition and evaluation of up to six samples.

This technical note describes some of the typical quality control tests and specifications for Agilent 2100 Bioanalyzer Protein 200 Plus LabChip Kits.

This compendium describes the applications of the Agilent LC/MSD TOF technology in the pharmaceutical industry, focusing on the advantages of high resolution, high accuracy, and wide dynamic range TOF mass spectrometry in drug development.

This paper mainly introduces the method of using Agilent Prep reverse phase column to realize the gradient separation of corticosteroids. Different diameter Agilent Prep reverse phase columns show nearly linear scale-up for the gradient separation of corticosteroids. With as much as 8 mg of total corticosteroid injected onto a 4.6 mm column and 320 mg onto a 30 mm column, the loadability of the columns is quite good.

This article describes a method for increasing sample load in preparative liquid chromatography by using DMSO as the injection solvent.

Agilent Reversed Phase Prep columns demonstrated both good loadability and good scalability for calcium channel blockers using gradient conditions. The columns also dis- played good resolution at all dimensions and allowed a linear scaleup from analytical to preparative dimensions

This application note demonstrates the identification and confirmation of degradation products, which have emerged in a formulation trial of the antibiotic drug amoxicillin under non-optimal conditions.

DuraGuard Columns: GC Columns with Built-In Protection

The document describes the metabolism of drugs in the body and the impact of enzyme activity on the formation and excretion rate of metabolites. The CYP3A4 and CYP2D6 enzyme families are considered the most important in this activity. The document uses dextromethorphan as a probe for the activity of the CYP2D6 metabolic phenotype and describes the metabolism and excretion of the drug in different metabolic types of patients. Additionally, the document mentions that some individuals have multiple copies of the gene that produces the CYP2D6 enzymes, leading to high metabolic rates of many drugs in these individuals and rendering the drugs therapeutically ineffective.

This document describes experimental modifications to inhibit ionization suppression when using LC-ESI-MS in negative ion mode for steroid estrogens and acidic herbicides. It introduces the problem of ionization suppression and methods to reduce its effects. The application of matrix clean-up in water samples to improve the apparent extraction efficiency of the analyte of interest when using LC-ESI-MS in negative ion mode is also discussed.

This article investigates the structure identification and confirmation of degradation products of the antibiotic drug amoxicillin. Accurate mass measurement and empirical formula confirmation for the compound ions and CID fragments were performed using ESI TOF.

This application note describes how the attributes of Time of Flight and Trap mass spectrometry can be combined to provide a highly reliable identification for an unknown compound. Time of Flight mass spectrometry provides a very accurate mass for the parent compound, and Trap mass spectrometry provides fragmentation data; together they greatly diminish the number of candidate compounds, and increase the confidence in a confirmation.

This application note describes the use of the Agilent 2100 bioanalyzer for stringent RNA quality control and the advantages of this method for high-resolution analysis of small and very small RNA samples.

This application note describes the preparative isolation of corticosteroid degradation products using mass-based fraction collection.