Agilent Manuals

This application note describes a method for performing copy number analysis of archived FFPE tumor samples using Agilent Oligo Array CGH. The method uses Kreatech’s Universal Linkage System (ULS™) technology, a non-enzymatic direct labeling methodology, that can be used with degraded FFPE tissue-derived DNA. The method also features a simple, single-step sample preparation procedure, which reduces the cost of sample preparation. In addition, the method employs a novel labeling strategy that significantly improves the labeling efficiency of samples. Finally, the method is validated using a variety of FFPE tissue samples, and the results demonstrate that the method is capable of producing high-quality copy number data.

This application note describes a rapid method for detecting and quantifying mycotoxigenic fungi using the Stratagene Mx3000P QPCR system.

This Application Note describes a method for the quantifi cation of two antibacterial drugs, olaquindox and carbadox in animal feedstuff using the Agilent 1260 Infinity Binary LC system.

This Application Note compares two suppliers Beckman Coulter and Advanced Analytical for separation matrices for protein characterization by capillary gel electrophoresis (CGE) with UV detection on the Agilent 7100 CE system. Two dif- ferent sample sets were analyzed: fi rst, a protein size standard and BSA as a test protein for molecular weight determination; second, a reduced antibody standard for quantifi cation of light chain, non-glycosylated heavy chain and heavy chain. To get an estimate for the intermediate precision, these experiments were performed with two different gel lots and capillary batches each. Across these experiments, the gels from both suppliers showed a similar performance. Impurity detection experiments were done with a low molecular weight protein spiked into a non-reduced antibody standard sample. With the gels of both suppliers it was possible to detect this protein down to a level of 0.1 %.

The Precision Optical Performance AlInGaP LED lamps provide superior light output for excellent readability in sunlight and are extremely reliable. These LED lamps are untinted, nondiffused, T-13/4 packages incorporating second generation optics producing well defined spatial radiation patterns at specific viewing cone angles. These lamps are made with an advanced optical grade epoxy, offering superior high temperature performance.

HDSP-5082 series of common cathode LED displays are high brightness, low power and large viewing angle LED displays. The series products adopt high quality AlGaAs red, HER red, yellow and green light emitting diodes, which have uniform illumination and good contrast. The series products have many different packages, including 7.6mm, 10.9mm and 16.2mm, to meet different application needs.

This document introduces the features of Agilent HDSP-511x/513x series LED seven-segment displays, including industry standard size, industry standard pinout, optional colors, excellent appearance, design flexibility and suitable range.

This note describes the analysis of three samples of polyethylene with the Agilent PL-GPC 220 instrument by gel permeation chromatography (GPC) and viscometry.

This application note describes the method for analysis of melamine resin with Agilent PLgel columns and gel permeation chromatography (GPC).

This document describes the analysis of frying fat using Agilent PLgel columns and gel permeation chromatography. By separating compounds of different molecular sizes, the content of high molecular weight substances in fats can be determined, thereby evaluating the quality and properties of the fats.

This document discusses the assessment of the purity of frying fats using Agilent PLgel and gel permeation chromatography. The analysis involves the separation of oligomeric glycerides based on molecular size in solution, allowing for the separation of the major component (monoglyceride) from the minor components (diglyceride, triglyceride) of frying fat. Quantitative analysis can then be performed to obtain information about the purity of the monoglyceride.

Rapid analysis of pesticides in vegetables by Agilent 5975T LTM GC/MSD and TSP

This application note shows the analysis of Aceton, n-Hexane, Methyl iso-Butyl Ketone (MIBK), Methyl iso-Butyl Carbinol (MIBC), and Methyl n-Butyl Ketone (MNBK, 2-Hexanone) using the Agilent 490 Micro GC.

This application note describes the benefits of the Agilent 2100 bioanalyzer in real-time quantitative PCR (QPCR) experiments. The results show that the Agilent 2100 bioanalyzer is an indispensable tool to ensure experimental success by verifying RNA template quality prior to QPCR experiments and to improve the assay design and validation process by monitoring size and purity of the QPCR amplicons.

This application note describes the applications and experimental considerations for FastMAS and UltraFastMAS techniques. FastMAS and UltraFastMAS techniques enable experiments with rotation speeds of 40 to 65 kHz, which reduces linewidths and suppresses spin diffusion caused by proton-proton dipolar interactions. These techniques enable experiments such as direct proton observe or solid-state HETCOR to collect spectra comparable to those that formerly required both spinning and experimentally difficult multiple-pulse homonuclear decoupling sequences. Another advantage of FastMAS and UltraFastMAS techniques is that they suppress spin diffusion between protons, allowing experiments to be conveniently performed on individual protons in single or multiple dimensional experiments.