Agilent Manuals

This document describes the analysis method for the pharmaceutical compound clozapine, used for the treatment of severely ill schizophrenic clients. The use of Zorbax SPE C18 EC cartridge significantly reduces levels of interfering substances in the bloodstream, allowing for accurate quantification of clozapine or clozapine-like compounds.

This is a document that introduces the features of the product.

This article presents a method for quantitatively analyzing opiates in urine using liquid chromatography/mass selective detector time-of-flight (LC/MSD TOF). The method utilizes accurate mass measurement to enhance selectivity.

The 5975 inert MSD – Benefits of Enhancements in Chemical Ionization Operation Technical Note This Technical Note describes the complete integration of chemical ionization (CI) on the 5975 inert MSD (mass selective detector), which now makes all the power of CI mass spectrometry (MS) conveniently available. CI has been fully automated by providing auto-tuning with electronic control of the reagent gases. All modes of MS operation in a single sequence, completely free of operator intervention, are now possible. Analytes can be sensitively determined in samples by implementing alternating positive- or negative-CI (PCI or NCI) modes. On the 5975 inert MSD, CI is as easy as EI (electron impact ionization).

This application note describes how the Agilent 2100 bioanalyzer can be used for the detection of a point mutation in the human prothrombin gene. The prothrombin protein plays a key role in blood clotting. A single nucleotide polymorphism (SNP), i.e. a point mutation in the prothrombin gene results in a common hereditary predisposition to venous thrombosis. We present a convenient and reliable mutation detection method by a PCR-based assay including a specific restriction digest (PCR-RFLP). Due to its high sensitivity and resolution, the applied microfluidic assay proves to be extremely suitable for detec- tion of the obtained PCR products and restriction fragments. The developed method is suitable for the workflow at Laborigo molecular diagnostic laboratory.

This document provides a general method for using finite element analysis in engineering modeling tools and system simulation tools. The goal of this method is to provide a structured method for creating finite element models and integrating them into larger engineering modeling and system simulation systems.

This document describes the different levels of instrument control with chromatography data systems and their implications for compliance. It provides a checklist for evaluating the capability of instrument control and data handling software.

This application note describes the possibilities of networking computers in an analytical laboratory using Agilent instrumentation and data systems. The benefits of networking are introduced, as well as how to use the IEEE 802.11b standard to achieve wireless network connectivity.

This document introduces the automatic method development of Agilent 1100 Series HPLC systems, Agilent ChemStation software and ILS ChromSmart MD software.

This is an article about the automated method development of Agilent 1100 Series HPLC systems. The article introduces the use of ChromSword Auto software in Agilent 1100 Series HPLC systems and Agilent ChemStation software.

Laboratories can improve the efficiency of their operations and ensure the accuracy of their data reporting, by automating the transfer of information between their chromatography data system (CDS) and their laboratory information management system (LIMS). In addition, the security of their chromatography data is enhanced when this automation is accomplished within the security framework established by regulatory guidelines such as the U.S. Food and Drug Administration’s ruling on electronic signatures and electronic records (21 CFR Part 11). This case study describes how Labtronics LimsLinkCDS enables laboratories to, smoothly and securely, integrate Agilent Cerity for Pharmaceutical QA/QC with any LIMS.

This paper introduces a method for the separation of basic drugs, such as ranitidine and paroxetine, based on hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization mass spectrometry (MS/MS) detection. The method showed good linearity over the 0.5–100 ppb levels. A spiked serum was cleaned up by protein precipitation and an aliquot was directly injected into the HILIC/ESI-MS system. Recovery was found to be 98% for ranitidine and 79% for paroxetine at the 100 ppb level.