Agilent Manuals

The document describes the ability to identify and quantify compounds based on single and dual column analyses. Instruments used include Agilent Technologies 6890 with other Hewlett-Packard instruments and data systems. The document demonstrates the acquisition and analysis of combined GC/MSD and GC/FID data.

This document describes the characteristics of gas chromatograph-mass spectrometer, including electronic pressure control, fast GC furnace, etc., which makes gas chromatograph-mass spectrometer have extremely high productivity, and it can realize small volume sample injection and automatic data processing.

The analysis of pesticides in water, foods, and soil is important worldwide. This document discusses the changes and regulations in environmental monitoring in the United States, Europe, Asia-Pacific region, and Latin America. It highlights the challenges and limitations of using gas chromatography/mass spectrometry (GC/MS) for pesticide detection, and the reliance on gas chromatography with an electron capture detector (GC/ECD). It also introduces the new 6890/5973 GC/MS system, which offers lower detection limits and can meet the current demands of environmental monitoring.

This note provides a method for improving GC performance in order to perform trace level GC/MS analysis. The note summarizes suggestions for improving GC performance, including using high purity carrier gas, optimizing sample injection and using optimized GC columns.

This application note describes reproducible methods for sample preparation and HPLC analysis of PNAs in soil, barbecued meat and waste water, all with good recovery rates, and detection limits that exceed regulatory requirements.

This application note describes how to use microbore reversed-phase HPLC to separate peptide mixtures, to characterize proteins or to isolate peptides for further studies.

This application note describes the methods for measuring protein size and protein expression using the Agilent 2100 Bioanalyzer and Protein 200 LabChip® kit.

This article introduces the Statistical Peak Identification feature in Agilent Technologies' Cerity NDS for Chemical QA/QC, which addresses the complications in traditional peak identification algorithms caused by peak shifts, changes in concentration, and external forces. It provides a more robust quantitation.

Orthogonal methods for protein purification and purity analysis are important to determine with certainty protein identity and purity. The Agilent 1100 Series purification system and the Agilent 2100 bioanalyzer with the Protein 200 Plus LabChip® kit provide an ideal solution that ensures efficient and reliable protein purification and characterization.

This article describes the use of high-performance liquid chromatography (HPLC) for the analysis of fat-soluble vitamins. HPLC can separate and detect these compounds at room temperature while blocking oxygen and light. UV-visible diode-array detection provides both qualitative and quantitative data. Electrochemical detection is also a highly sensitive and selective HPLC method. Different food matrices require different extraction procedures, including alkaline hydrolysis, enzymatic hydrolysis, alcoholysis, direct solvent extraction, and supercritical fluid extraction.

This document describes the collection of fractions from an oligonucleotide separation using gel electrophoresis and offline MALDI-TOF analysis. By using the Agilent oligonucleotide kit and Agilent Capillary Electrophoresis system, antisense oligonucleotides can be successfully separated. The Agilent ChemStation software allows for fully automated collection of fractions using signal A as the peak sensor. This method enables further analysis of the separated peaks and determination of the molecular mass.

This document introduces the detailed steps for analyzing testosterone and its metabolites using HPLC.

The HPLC method presented here shows the chromatogram of the four common antiasthmatic drugs theophylline, theobromine, caffeine and emprofylline using isocratic analysis on a reversed phase column and UV detection.