Agilent Manuals

Introduced 1.8-µm Totally Porous particles manufactured by Agilent Technologies, which can achieve the expected resolving power and efficiency of a 150-mm, 5-µm column in a 30-mm configuration.

This document introduces the Agilent ZORBAX Rapid Resolution High Throughput HPLC columns using 1.8 µm particles that provide outstanding efficiency in short column lengths for high sample throughput of liquid chromatography and liquid chromatography/mass spectrometry methods. These columns can be used for either isocratic or gradient analyses with high quality, reliable results.

This Application Note details how the separation of enantiomers, stereoisomers or diastereomers can be improved by recycle chromatography. The configuration of the system using two identical columns and a 2-position/6-port valve is shown, and the scope and limitations of the system are explained. An application example, which demonstrates the improvement in purity of the collected fractions when using recycle chromatography, is described.

Laboratory projects that require accurate analysis of PCR products can be divided into two classes: low throughput and high throughput.

Zorbax Rapid Resolution HT columns can reduce analysis time and improve resolution.

The U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID) has established a database entry for Bacillus anthracis using the Sherlock® Microbial Identification System (MIS) from MIDI, Inc. The Sherlock MIS identifies over 1,500 species of bacteria, including six bacteria considered potential agents of biological terrorism, through gas chromatographic analysis of fatty acid methyl esters (FAMEs). The new B. anthracis data set entry in the Sherlock Bioterrorism Library allows for accurate identification of B. anthracis isolates, estimation of strain relatedness, and potential forensic analysis for attribution of biological threat agent use.

This paper presents a method for determining sub-ppb levels of perchlorate anion using ion chromatography/mass spectrometry (IC/MS). The IC/MS method follows EPA Method 314 methodology originally developed for IC with conductivity detection at the single ppb range [1]. The IC/MS method is not affected by the addition of the interference matrix described in Method 314 on perchlorate recoveries throughout the measurable range. Typical recoveries are 90%–105% at the 0.5 and 1-ppb level in synthetic drinking and waste waters with the method detection limit (MDL) less than 100 ppt.

This document describes a routine method for the quantitative determination of 4-nonylphenol (NP), 4-nonylphenol monoethoxylate (NP1EO), and 4-nonylphenol diethoxylate (NP2EO) in sludge samples. A Soxtec® extraction procedure was used to enrich the target compounds from the solid matrix. Quantitative determinations were performed by high-speed gas chromatography/mass spectrometry using a short apolar fused silica column. Derivatization allows NP1EO and NP2EO to be analyzed by gas chromatography. The relative standard deviation was close to 5% for the analysis of 10 different sludges analyzed seven times each. Recoveries were determined for a sludge reference material and were higher than 90%. The experimental limits of quantification were 2, 5, and 5 µg/g of dry matter (µg/g DM), respectively, for NP, NP1EO, and NP2EO. Good agreement was observed between results obtained with this method and those obtained by our previous method, which used normal-phase liquid chromatography with an aminosilica column. This method was applied to the determination of alkylphenols and alkylphenol mono- and diethoxylates in sewage sludge by high-speed gas chromatography/mass spectrometry.

This Application Note describes the optimization of the Agilent 1100 Series high throughput LC/MS system for different analytical tasks. It provides detailed instructions on how to optimize chromatography, diode-array and MSD detection to achieve highest sample throughput in combination with substance identification, how to acquire quantitative information while maintaining very high sample throughput, and how to achieve fast analysis of very low sample amounts.

Mass-based fraction collection of compound libraries using the Agilent 1100 Series purification system

The paper introduces the application of LC/MS in drug monitoring, which can be used to analyze over 200 drugs, including identification and quantification.

This article discusses the application of mass spectrometry technology in protein research, focusing on the advantages and challenges of electrospray ionization combined with time-of-flight mass spectrometer for protein identification.