Agilent Manuals

This application note demonstrates the configuration of the Agilent 1200 Series RRLC AS purification system and the use of an optimized method for purifying dye-labeled or Fmoc-protected peptide nucleic acids from solid-phase synthesis. It provides an overview of the characteristics and applications of peptide nucleic acids.

This document describes the application of the Agilent 1100 Series 12-position/13-port valve in compound isolation and purification in the pharmaceutical industry. The system is optimized for high throughput, capacity, and productivity. This application note demonstrates how the Agilent 1100 Series valve can enhance the purification system by adding easy recovery collection and simple time-based fraction collection.

To increase throughput in preparative HPLC, it is necessary to load high amounts of sample onto the column. This application note describes two common techniques - sandwich injection and organic-phase injection, and how they can be performed easily on an Agilent 1100 Series purification system.

This Application Note describes a more sophisticated approach to peak-based fraction collection by using the parameters up and down slope, upper threshold and maximum peak duration.

This article discusses the importance of impurity isolation and purification in impurity profiling using the Agilent 1200 Series LC system, and elucidates the structures of impurities through NMR analysis. By optimizing the method and conducting loading experiments, the impurities were successfully isolated from the crude sample with high purity and recovery. Sufficient amounts of each compound were obtained through repetitive injections for further structural elucidation.

This Application Note describes a method for generating a small method set based on retention time windows in the pre-preparative analytical run. Using Agilent standards with known elution properties makes this an easy four-step process, which can be accomplished in less than one hour. An application example demonstrating how the resolution of the target compound can be improved is also shown.

This Application Note describes the isolation of three antianginal drugs (nifedipine, nisoldipine, and nimodipine) from a mixture as an example application for purification of pharmaceutical drugs using the Agilent 1100 Series purification1,2 system PS (preparative scale). The goal of the application was to purify 20 mg of each compound in a single run and to get three fractions containing only the desired substances.

These single, dual and quad channel, hermetically sealed optocouplers provide military-grade reliability and high performance in a wide variety of harsh industrial applications.

This document describes how to accelerate the entire method development process by using Agilent Rapid Resolution HT columns in combination with the Agilent 1200 Series Rapid Resolution LC (RRLC) system.

This Application Note demonstrates efficient methods for conserving acetonitrile using Agilent's ZORBAX columns as a solution to the global acetonitrile shortage.

This application note describes the method for generating non-linear ELSD calibration curves using ChemStation software and the quantification of non-UV active compound topiramate using this curve.

Using the Agilent 1200 Series Rapid Resolution LC system and Agilent Method Translator software, USP pravastatin sodium chromatographic purity method is used to conduct a fast LC analysis. This application note describes how to start from a USP method, incorporate the allowed adjustments, and produce a fast method that meets system suitability requirements.

This Application Note describes the use of the Agilent 1120 Compact LC to analyze antioxidants in chewing gum.