Agilent Manuals

Agilent Technologies' new, fast GC/MSD method can significantly speed up the screening of pesticides. Agilent's GC Method Translation software was used in developing the new method based on the standard 42-min method. A 15 m × 0.25 mm × 0.25 mmmmm Agilent HP-5MS column was used to increase analysis speed up to fourfold. The time savings were implemented in increments (down to 10.5 minutes) to verify the predictability of scaling and the effect of scaling on the signal-to-noise ratio.

A new instrumental method for the determination of 29 phthalate esters, including six recently banned from baby toys by the European Union, using positive chemical ion- ization and retention-time locking is described.

The document describes the use of liquid chromatography-mass spectrometry (LC-MS) for the analysis of organophosphate pesticides. Compared to existing gas chromatography-mass spectrometry (GC-MS) and ultraviolet (UV) detection methods, LC-MS simplifies sample cleanup procedures, reduces analysis time, and method development time. By using an electrospray ion (ESI) source, this method avoids the thermal degradation of labile pesticides encountered in GC and eliminates the need for preliminary derivatization to increase compound volatility.

This document describes a method for extracting 壬基酚 from water samples using the AccuBOND II ODS (C18) 6 mL/500 mg cartridge.

This document describes the application of the Agilent 2100 bioanalyzer with the DNA 500 LabChip® kit for the development of meat speciation assays. By using the bioanalyzer to confirm the suitability of primer sets before probe production, the cost of probe production can be reduced.

This paper describes a method for analyzing triglycerides using liquid chromatography/mass spectrometry. By employing atmospheric pressure chemical ionization in positive ion mode, the composition of triglycerides can be analyzed quickly and accurately. This method requires simple sample preprocessing without saponification and offers high sensitivity and separation capacity.

Sugars and sugar alcohols were successfully analyzed by negative ion mode of liquid chromatography/mass spectrometry using atmospheric pressure chemical ionization

This document introduces the method for the detection of 3-chloro-1,2-propanediol (3-MCPD) in hydrolyzed vegetable protein.

This document describes a method for quantifying pesticides in the parts-per-trillion range using retention time locking and GC/MS application. By using a selected ion monitoring method and large volume injection capability, the target compound quantitation limit can be lowered to the low parts-per-billion (pg/µL) range. Additionally, an automated 567-compound retention time locking pesticide mass spectral library can screen an acquired sample's data file for all 567 compounds in seconds. The library can also be used for rapid screening of samples acquired in selected ion monitoring method. Using the compound library information, a selected ion monitoring method for 80 target compounds was created in less than 2 hours without running any analyses.

This application note describes the use of the Agilent 7500c ICP-MS system to measure major, minor and trace elements in two standard reference plant materials.

This document describes the use of liquid chromatography/mass selective detection (LC/MSD) technique to replace the nonspecific post-column derivatization process for detecting low ppb levels of carbamate pesticides in food. System linearity, sensitivity, and repeatability were evaluated on nine carbamates, and the instrument detection limits were determined to be about 3 to 10 ppb for all the analytes in broccoli extract. This method overcomes the false positive issue of fluorescence detection method, providing higher specificity, no additional hardware and plumbing requirements, and lower limits of detection.

Methods for detecting genetically modified organisms (GMOs)

This note describes the results of using hydrogen carrier gas and increasing the oven ramp rate in the analysis of chlorinated pesticides and herbicides using DB-35ms and DB-XLB columns.

Rapid Analysis of CLP Pesticides Using High-Temperature DB-35ms and DB-XLB Columns

This application note describes two retention time locked methods and retention time databases that improve the analysis of fatty acid methyl esters. In the first method, fatty acid methyl esters are analyzed on a DB-Wax column using gas chromatography/flame ionization detector or gas chromatography/mass spectrometry. In the second method, a DB-23 column is used. Retention time and mass spectral libraries are available for both methods. Retention time locking allows easy peak identification, easy exchange of data between instruments (gas chromatography/flame ionization detector, gas chromatography/mass spectrometry, different labs), and avoids the need to modify the retention times in calibra- tion tables after column maintenance or column change.