Agilent Manuals

This is an application note that describes how to use Agilent's Polaris C18-A column to separate anti-inflammatory and analgesic (acidic) drugs.

Describes a method for the analysis of polycyclic aromatic hydrocarbons (PAHs) using the Varian 920-LC and Pursuit™ 3 PAH Column

This article introduces a simple scale-up method for a 940-LC analytical to preparative HPLC using a linear scale-up factor to change flow rate, column size and sample injection volume to produce an effective preparative HPLC separation.

This article describes a HPLC analysis method for the USP assay of ibuprofen and the limit test of ibuprofen-related compound C in ibuprofen tablets using Varian 920-LC liquid chromatograph and Varian Pursuit C18 column. The experimental results show that this method has high sensitivity and selectivity and can be used for rapid analysis of ibuprofen-related compound C.

This article presents a method for the USP assay of ibuprofen oral suspension using Varian 920-LC and Pursuit™ XRs C8 column. The results obtained from the experiment show that all performance requirements of the USP methods are satisfied and demonstrate a significant enhancement in laboratory productivity when using this system.

This document describes a high-performance liquid chromatography method that uses fluorescence detection following post-column derivatization for enhanced sensitivity and selectivity. This method has become the method of choice for the analysis of carbamate residues in both food and water.

This article presents the experimental results of analyzing phenoxyacid herbicides and bentazone in water samples using automated on-line solid-phase extraction. The method can be used for frequent monitoring of contaminants in the river Rhine and drinking water samples.

This document introduces the application of using Varian Star 9080 EC Detector to analyze phenol in water

This document describes the method of using the Varian Star 9080 Amperometric Electrochemical Detector for pulsed amperometric detection of disaccharides. The document provides detailed information on the instrument, column, flow rate, mobile phase, sample, temperature, and flow cell parameters used.

Application note: Analysis of amino acid neurotransmitters with Star 9080 EC detector

The document introduces the application of Star 9080 Amperometric Electrochemical Detector . The detector is used to analyze the standards of 30 nmol/L noradrenaline (1), adrenaline (2) and dopamine (3).

This application note describes a method for rapid separation and quantitation of a mixture of 10 non-steroidal anti-inflammatory drugs (NSAIDs) using fast high-performance liquid chromatography (HPLC) and diode array detector (DAD). Separations are performed in less than 5 minutes using a 3-cm long column. To enhance resolution, two wavelengths, 254 and 360 nm, are used. Quantitation is performed by integrating the peak areas of the absorbance peaks at each wavelength. This method can be used to rapidly identify and quantify the NSAIDs present in a mixture.

This application note describes the use of a fully automated on-line LC-GC system for the clean-up and determination of PAH in urban air particulate extracts. The system consists of a high-performance liquid chromatograph (LC) coupled on-line to a gas chromatograph (GC).

This article describes the method of quantitation of partially resolved peaks using multicomponent analysis (MCA) in high-performance liquid chromatography (HPLC) with diode array detection. MCA allows accurate quantitation and identification of unknown components even under partial or zero resolution conditions. It also provides a more thorough assessment of peak homogeneity compared to other purity assessment techniques. The method enables quantitation of small peaks fused to a major peak, even when they are not visually detected or integrated separately, without the need to select different optimum wavelengths to distinguish the compounds.