Instruction manual for Takara TB Green Premix Ex Taq II (Tli RNaseH Plus) reagent kit, covering usage, storage, and instrument compatibility.
This manual describes the TB Green Premix Ex Taq II (Tli RNaseH Plus) reagent kit, a 2× premix for real‑time PCR that includes TB Green fluorophore and Tli RNaseH to improve specificity and quantitation. The kit provides 50 µl reactions for up to 200 runs and is compatible with a range of authorized real‑time PCR instruments.
The document includes product description, principle of the kit, contents, storage conditions, required reagents and instruments, safety precautions, detailed operation steps, and guidance for selecting experimental conditions. It also lists related Takara products and regulatory information. This manual is written in Chinese.
| Concentration | 2× premix |
|---|---|
| Enzyme | TaKaRa Ex Taq HS with Hot Start |
| RNase H | Tli RNaseH (heat‑stable) |
| Storage temperature (long term) | -20 °C |
| Shelf life at 4 °C | 6 months |
| Reactions per kit | 200 × 50 µl |
Store the kit at –20 °C for long‑term preservation.
The kit provides 200 reactions of 50 µl each.
Gently invert the tube up and down; do not vortex or shake vigorously.