This is an instruction manual that introduces the product characteristics and usage methods of LumiSensorTM Plus Chemiluminescent HRP Substrate Kit.
The LumiSensor™ Plus Chemiluminescent HRP Substrate Kit is designed for detecting horseradish peroxidase (HRP) on immunoblots, such as Western and Dot blots. The kit contains two reagents, Reagent A and Reagent B, sufficient for 10 mini gel detections or 600 cm² of membrane. It is noted for being easy to perform with a quick three-minute incubation period, offering high sensitivity and low background results.
| Reagent Volume | 30 ml per reagent |
|---|---|
| Detection Capacity | 10 mini gel or 600 cm² membrane |
| Incubation Time | 3 minutes |
| Storage Temperature | 2 to 8°C |
| Stability Period | 12 months |
| Product Code | L00225 |
The procedure is quick and simple, with only a three-minute incubation period.
Store the kit at 2 to 8°C to ensure stability for 12 months.
Yes, it is compatible with and highly recommended for GenScript One-Step Western™ Advanced Kits.
Check if there is too little protein loaded or if the transfer efficiency is poor.