Technical manual for GenScript Protein G Resin L00209, detailing characteristics, purification procedures, application examples, and troubleshooting for IgG…
This document serves as a technical manual for GenScript Protein G Resin (Cat. No. L00209), a recombinant streptococcal protein G immobilized on agarose media. It is designed for the purification and isolation of mammalian IgGs, offering high affinity for specific subclasses like human IgG3, mouse IgG1, and rat IgG2a, while lacking albumin binding sites to reduce nonspecific binding.
| Matrix | spherical agarose, 4% |
|---|---|
| Average particle size | 90 μm (45–165 μm) |
| Static binding capacity | >20 mg sheep IgG/ml drained medium |
| Storage condition | 20% ethanol at +4 to +8 °C |
| Number of IgG binding sites per ligand | 3 |
| MW of ligand | Approximately 22 kDa |
| pI of ligand | 4.69 |
It has greater affinity for human IgG3, mouse IgG1, and rat IgG2a.
No, unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.
Columns can be regenerated up to 10 times without significant loss of binding capacity.
Wash the column with 5 ml of PBS containing 0.02% sodium azide and store at 4°C.