GenScript L00847 SARS-CoV-2 Surrogate Virus Neutralization Test Kit User Manual

About this manual

The GenScript SARS-CoV-2 Surrogate Virus Neutralization Test (sVNT) Kit is designed to detect circulating neutralizing antibodies that block the interaction between the viral spike glycoprotein receptor binding domain (RBD) and the ACE2 cell surface receptor. The assay utilizes a blocking ELISA principle involving HRP-conjugated RBD and human ACE2 protein.

What's inside

• Intended Use
• Assay Principle
• Kit Contents
• Storage
• Specimen Collection
• Protocol
• Quality Control
• Interpretation of Results

Frequently asked questions

What is the minimum volume required for a serum or plasma sample?

A minimum volume of 50 μL per serum or plasma sample is recommended.

What temperature should reagents be equilibrated to before use?

All reagents must be allowed to equilibrate to room temperature (20° to 25°C) before running the assay.

What is the incubation time for the neutralization reaction?

The mixture should be incubated at 37°C for 30 minutes.