This is a technical manual for CHO-K1/MT2/Gα15 cell line. The cell line is used for functional assay of MT2 receptor.
This manual provides background on the human MT2 melatonin receptor and details the characteristics of the stable CHO‑K1/MT2/Ga15 cell line offered by GenScript. It includes catalog information, gene details, and recommended culture media.
The document describes step‑by‑step protocols for thawing, subculturing, and maintaining the cell line, as well as the limited‑use license agreement that governs its use in research settings.
| Catalog Number | M00312 |
|---|---|
| Cell Line Name | CHO-K1/MT2/Ga15 |
| Gene Synonyms | MTNR1B |
| Expressed Gene | Genbank Accession Number NM_005959 |
| Quantity | 2 vials (3×10⁶ per vial) frozen cells |
| Stability | 16 passages |
| Application | Functional assay for MT2 receptor |
| Freeze Medium | 45% culture medium, 45% FBS, 10% DMSO |
| Complete Culture Medium | Ham’s F12, 10% FBS |
| Culture Medium (with antibiotics) | Ham’s F12, 10% FBS, 400 µg/ml G418, 100 µg/ml Hygromycin B |
| Mycoplasma Status | Negative |
| Storage | Liquid nitrogen immediately upon delivery |
Remove the vial from the liquid‑nitrogen tank and thaw quickly in a 37 °C water‑bath, then decontaminate the outside with 70 % ethanol before transferring to growth medium.
Add Hygromycin B to 100 µg/ml and G418 to 400 µg/ml the day after thawing.
The product is non‑transferable and may only be used at the site of purchase; it cannot be sold or used for human consumption or clinical applications.