CHO-K1/CRF2/Gα15 cell line is a cell line stably expressing human CRF2 receptor and Gα15 protein, which can be used for functional assay of CRF2 receptor
This manual introduces the human recombinant corticotropin‑releasing factor receptor 2 (CRF2) stable cell line produced by GenScript. It provides background on the GPCR, gene information, and the intended application for functional assays.
The document details practical protocols for thawing the frozen vials, subculturing the cells, recommended media compositions, selection antibiotic concentrations, and storage conditions. It also includes representative calcium‑response data, references, and the limited‑use license agreement governing the product.
| Catalog Number | M00208 |
|---|---|
| Cell Line Name | CHO-K1/CRF2/Ga15 |
| Gene Synonyms | CRF2, CRHR2, CRFR2, CRH2 |
| Expressed Gene Accession | NM_001883 |
| Host Cell | CHO-K1 |
| Quantity | 2 vials (3×10⁶ per vial) frozen cells |
| Stability | 16 passages |
| Application | Functional assay for CRF2 receptor |
| Freeze Medium | 45% culture medium, 45% FBS, 10% DMSO |
| Culture Medium | Ham’s F12, 10% FBS, 400 µg/ml G418, 200 µg/ml Hygromycin B |
| Mycoplasma Status | Negative |
| Storage | Liquid nitrogen immediately upon delivery |
Add Hygromycin B to 200 µg/ml and G418 to 400 µg/ml the day after seeding.
The cell line remains stable for up to 16 passages.