This document describes the technical manual for the human recombinant κ-opioid receptor OPRK1 stable cell line, including the characteristics and features of the cell line.
This manual provides an overview of the human recombinant κ‑opioid receptor OPRK1 stable cell line (catalog number M00290) produced by GenScript. It describes the cell line background, gene information, and typical applications such as functional assays for the OPRK1 receptor.
Detailed protocols for thawing, subculturing, and maintaining the CHO‑K1/OPRK1/Gα15 cells are included, along with representative calcium‑flux data, storage conditions, and a limited‑use license agreement for research use only.
| Catalog Number | M00290 |
|---|---|
| Cell Line Name | CHO‑K1/OPRK1/Gα15 |
| Quantity | 2 vials (3×10⁶ per vial) |
| Stability | 16 passages |
| Application | Functional assay for OPRK1 receptor |
| Freeze Medium | 45% culture medium, 45% FBS, 10% DMSO |
| Complete Culture Medium | Ham’s F12, 10% FBS |
| Culture Medium (with selection) | Ham’s F12, 10% FBS, 100 µg/ml Hygromycin B, 200 µg/ml Zeocin |
| Mycoplasma Status | Negative |
| Storage | Liquid nitrogen immediately upon delivery |
Remove the vial from the liquid nitrogen tank and thaw the cells quickly in a 37 °C water‑bath.
Add Hygromycin B to 100 µg/ml and Zeocin to 200 µg/ml the following day.
The catalog number is M00290.
Store the vials in liquid nitrogen immediately upon delivery.