The GenScript Anti‑DYKDDDDK G1 Affinity Resin Technical Manual provides usage instructions, buffer recipes, elution methods, reagent compatibility, and…
This manual describes the Anti‑DYKDDDDK G1 Affinity Resin, a 4% cross‑linked agarose resin functionalized with a mouse monoclonal antibody (clone G1) for purification of DYKDDDDK‑tagged proteins from bacteria, yeast, or mammalian cells. It lists product characteristics, storage conditions, and reuse limits.
The document details required equipment, step‑by‑step procedures for column, batch, and immunoprecipitation formats, multiple elution strategies (acid, alkaline, neutral, competitive peptide, PAGE sample buffer), reagent compatibility tables, example applications, and a troubleshooting guide.
| Product content | 50% settled resin in TBS with 0.02% sodium azide |
|---|---|
| Matrix | 4% cross‑linked agarose |
| Average bead size | 90 μm |
| Binding capacity | ≈1 mg DYKDDDDK‑tagged protein per ml settled resin |
| Storage temperature | 2‑8 °C (up to 12 months) |
| Resin reuse | At least 4 times (up to 10 times with proper maintenance) |
Store the resin at 2‑8 °C for up to 12 months and do not freeze it.