This is a technical manual for CHO-K1/ADRB2/Gα15 cell line, including cell line name, gene sequence, application, freezing conditions, culture medium, application, culture method, inventory, etc.
This manual describes GenScript's human recombinant ADRB2 adrenoceptor stable cell line (CHO-K1/ADRB2/Ga15). It provides background on the beta‑adrenergic receptor family, the cell line’s genetic details, and its intended use for functional assays of the ADRB2 receptor.
The document includes representative calcium‑flux data, step‑by‑step thawing and subculturing protocols, storage recommendations, and the Limited Use License Agreement governing the product. It also lists key specifications such as catalog number, medium composition, and mycoplasma status.
| Catalog Number | M00308 |
|---|---|
| Cell Line Name | CHO-K1/ADRB2/Ga15 |
| Expressed Gene (GenBank) | NM_000024 |
| Quantity | 2 vials (3×10⁶ per vial) frozen cells |
| Stability | 16 passages |
| Mycoplasma Status | Negative |
| Freeze Medium | 45% culture medium, 45% FBS, 10% DMSO |
| Culture Medium | Ham’s F12, 10% FBS, 200 µg/ml Zeocin, 100 µg/ml Hygromycin B |
Remove the vial from liquid nitrogen, thaw quickly in a 37 °C water‑bath, decontaminate the outside with 70 % ethanol, transfer to a tube with complete growth medium, centrifuge at 200 × g for 5 min, discard the medium and resuspend the cells in fresh complete growth medium.
A subcultivation ratio of 1:3 to 1:8 is recommended weekly.
The cell line is mycoplasma‑free (Negative).
Store the vials in liquid nitrogen immediately upon delivery.