Invitrogen ViraPower HiPerform Lentiviral Expression Systems User Manual

This document is the ViraPower™ HiPerform™ Lentiviral Expression Systems manual


Invitrogen ViraPower HiPerform Lentiviral Expression Systems User Manual - cover page
Brand
invitrogen
Document type
User Manual
Language
English
Pages
62
File format
PDF
File size
610 KB
Published
16 October, 2023
Updated
16 October, 2023
MD5 checksum
92DF54845543C2CCD189C60B7358FE87

About this manual

The ViraPower™ HiPerform™ Lentiviral Expression Systems allow the creation of a replication-incompetent, HIV-1-based lentivirus to deliver and express a gene of interest in dividing or non-dividing mammalian cells. The system utilizes four new expression vectors containing WPRE and cPPT elements to yield cell-specific, high-performance results with increased transgene expression and viral titer.

What's inside

  • Kit Contents and Storage
  • System Summary
  • Biosafety Features of the System
  • Experimental Outline
  • Generating Your pLenti Expression Construct
  • Producing Lentivirus in 293FT Cells
  • Titering Your Lentiviral Stock
  • Transduction and Analysis
  • Troubleshooting

Specifications

Transformation efficiency of Stbl3™ Cells≥ 1 × 108 cfu/μg plasmid DNA
Cell line content per vial3 × 106 frozen cells in 1 mL of Freezing Medium

Frequently asked questions

What is the intended use of these kits?

The products are for Research Use Only and are not intended for any animal or human therapeutic or diagnostic use.

What mammalian cells can this system deliver genes to?

The system allows delivery of a target gene to dividing and non-dividing mammalian cells.

What storage temperature is required for Lipofectamine 2000?

The reagent must be stored at 4°C and should not be frozen.

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