This manual provides instructions for cloning, transformation, transfection, storage, and purification using Invitrogen's pcDNA™3.1/V5-His A, B, and C vectors.
The document serves as a user guide for the Invitrogen pcDNA™3.1/V5-His A, B, and C vector system. These are 5.5 kb plasmid vectors derived from pcDNA™3.1(+) designed for high-level expression, purification, and detection of recombinant proteins in mammalian hosts. The vectors contain three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the V5 epitope and a polyhistidine metal-binding peptide.
| Vector Size | 5.5 kb vectors |
|---|---|
| Promoter | Human cytomegalovirus (CMV) immediate-early promoter |
| Storage Temperature | -20ºC |
| Concentration | 0.5 μg/μl |
| Volume per Vector | 40 μl |
Upon receipt, store vectors at –20ºC.
Use recA and endA deficient E. coli strains like TOP10, TOP10F’, DH5 α™-T1R, or DH10B™.