This document is about the use of pYES2/NT A, B, and C and pYC2/NT A, B, and C.
This document is a user guide for the Invitrogen pYES2/NT and pYC2/NT yeast expression vectors, which are designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae. The kit includes vectors with N-terminal tags for purification and detection, as well as control vectors containing the lacZ gene.
| Vector Size (pYES2/NT) | 6.0 kb |
|---|---|
| Vector Size (pYC2/NT) | 4.7 kb |
| Host Organism | Saccharomyces cerevisiae |
| Promoter | Yeast GAL1 promoter |
| Selection Marker (Yeast) | URA3 auxotrophic marker |
| Selection Marker (E. coli) | Ampicillin resistance gene |
| Replication Origin (pYES2/NT) | 2μ origin |
| Replication Origin (pYC2/NT) | CEN6/ARSH4 sequence |
| Storage Temperature (Plasmid DNA) | -30°C to -10°C |
| Storage Temperature (Yeast Strain) | 2°C to 8°C |
They are designed for inducible expression of recombinant proteins in Saccharomyces cerevisiae.
Store the plasmid DNA at −30°C to −10°C.
pYES2/NT contains a 2μ origin for high copy replication, while pYC2/NT contains a CEN6/ARSH4 sequence for low copy replication.