This document provides a validation and assay performance summary for the Invitrogen CellSensor HSE-bla HeLa Cell Line (Cat. no. K1813), detailing its use…
The CellSensor HSE-bla HeLa Cell Line is an engineered HeLa cervical cancer cell line designed to monitor the heat shock response (HSR) and unfolded protein response (UPR) pathways. These cells feature a beta-lactamase reporter gene driven by a heat shock element (HSE) and are stably integrated via FACS isolation. The document outlines the biological mechanism where stress-induced activation of Heat Shock Factors (HSFs) leads to the upregulation of target genes like Hsp90 and Hsp70.
| Recommended cell number | 8,000 cells/well |
|---|---|
| Recommended DMSO concentration | up to 0.5 % |
| Stimulation Time | 5 hours |
| Max. [Stimulation] 17-AAG | 600 nM |
| Average 17-AAG EC 50 | 50 nM |
| Average Z'-Factor (EC 100) | 0.70 |
| Average Response Ratio | 10.1 |
The recommended cell number is 8,000 cells per well.
The recommended DMSO concentration is up to 0.5%.
The recommended stimulation time is 5 hours.
The average EC50 for 17-AAG under optimized conditions is 50 nM.