TrueCut™ Cas9 proteins are used for genome editing applications with CRISPR technology
This document provides a user guide for Invitrogen TrueCut Cas9 Proteins, specifically the v2 version, which are used for genome editing applications with CRISPR technology. The protein forms a stable ribonucleoprotein complex with guide RNA and includes nuclear localization signals to enhance delivery to the nucleus. It is designed for high editing efficiency and cell survivability compared to plasmid-based systems.
| Protein Type | Recombinant Streptococcus pyogenes Cas9 (wt) |
|---|---|
| Storage Temperature | -20°C |
| Recommended gRNA Ratio | 1:1 molar ratio |
| Catalog Numbers | A36496, A36497, A36498, A36499 |
For maximum efficiency in difficult-to-transfect cell types or for HDR knock-in editing, use the Neon™ Transfection System.
Use RNAse-free reagents, tubes, and barrier pipette tips while setting up experiments.
Use cells at 30–70% confluence on the day of transfection with lipid-mediated delivery.