This manual provides instructions for using the Qubit RNA Assay Kit with the Qubit 2.0 Fluorometer, including storage, calibration, and experimental protocols.
The Qubit RNA Assay Kits for use with the Qubit 2.0 Fluorometer provide concentrated assay reagent, dilution buffer, and prediluted RNA standards for accurate RNA quantitation. The assay is highly selective for RNA over double-stranded DNA and is accurate for initial sample concentrations ranging from 250 pg/µL to 100 ng/µL. The kit is designed to be performed at room temperature, with a signal stability of 3 hours.
The document details the experimental protocol, which involves preparing a working solution by diluting the reagent 1:200 in the provided buffer. Users must calibrate the Qubit 2.0 Fluorometer using two standards before measuring samples. The manual also covers critical parameters such as assay temperature (22–28°C), incubation time (2 minutes), and handling precautions for the reagents and standards.
| Sample volume range | 1 µL to 20 µL |
|---|---|
| Concentration range | 250 pg/µL to 100 ng/µL |
| Assay temperature | 22 –28˚C |
| Incubation time | 2 minutes |
| Signal stability | 3 hours |
| Reagent dilution ratio | 1:200 |
| Final volume per tube | 200 µL |
The assay is accurate for initial sample concentrations from 250 pg/µL to 100 ng/µL.
Tubes should be incubated at room temperature for 2 minutes after mixing the sample or standard with the working solution.
Yes, all Qubit assay kits can also be used with the Qubit 1.0 Fluorometer.