User manual for the Invitrogen EVOS FL Auto 2 automated live‑cell fluorescence imaging system.
The EVOS FL Auto 2 is a fully automated live‑cell fluorescence imaging platform that combines high‑performance optics, adjustable LED light cubes and dual‑camera imaging for fast, high‑throughput experiments. It supports bright‑field and up to four fluorescence channels, a motorized X/Y stage, and an on‑stage incubator for precise environmental control.
The system includes the Celleste image‑analysis software, interchangeable objectives (1.25‑100×), and a range of accessories such as container adapters and light‑cube modules, enabling flexible configuration for a wide variety of cell‑biology applications. This manual is written in Chinese.
| Light source | Adjustable LED light cube (life >50,000 h) |
|---|---|
| Fluorescence channels | 4 fluorescence light cubes + 1 bright‑field channel |
| Objective turret positions | 5 |
| Objective magnification range | 1.25–100× |
| Monochrome camera | 1.3 MP CMOS |
| Color camera | 3.2 MP CMOS |
| Stage travel | 120 mm × 80 mm, sub‑micron resolution |
| Dimensions (L×W×H) | 46 cm × 33 cm × 36 cm |
| Weight | 16 kg |
It uses an adjustable LED light cube with a lifespan of over 50,000 hours.
The system can accommodate four fluorescence light cubes plus one bright‑field imaging channel.
Objectives can be selected from 1.25× up to 100× magnification.
Yes, the EVOS Onstage stage‑top incubator controls temperature, humidity, CO₂ and O₂ for long‑term live‑cell experiments.