Technical guide for the Invitrogen NuPAGE electrophoresis system with protocols, gel specs, and troubleshooting.
The NuPAGE® Technical Guide provides detailed information on the NuPAGE® electrophoresis system, including gel specifications, buffer compositions, sample preparation, electrophoresis procedures, and staining methods. It also covers system components, recommended storage conditions, and safety warnings.
The guide includes troubleshooting tips, accessory product listings, and contact information for technical service, making it a comprehensive resource for researchers using NuPAGE® Bis‑Tris and Tris‑Acetate pre‑cast gels.
| Gel Matrix | Acrylamide/Bisacrylamide |
|---|---|
| Gel Thickness | 1.0 mm |
| Gel Size | 8 cm x 8 cm |
| Cassette Material | Styrene Copolymer (recycle code 7) |
| Sample Well Configuration | 1, 9, 10, 12, 15, 17‑well, 2D‑well, IPG‑well |
NuPAGE® Bis‑Tris gels should be stored at 4‑25 °C and NuPAGE® Tris‑Acetate gels at +4 °C.
The NuPAGE® Bis‑Tris gels have a shelf life of 12 months when stored at 4‑25 °C.
No, using NuPAGE® Bis‑Tris gels with MOPS or MES running buffer without SDS can cause excessive heat and poor resolution.