Invitrogen FastTrack 2.0 Kit mRNA Isolation Quick Reference Card

About this manual

This document serves as a quick reference card for the FastTrack 2.0 Kit, specifically for isolating mRNA from lysates of tissue culture cells or tissues. It provides a condensed set of instructions starting with lysate preparation, followed by oligo(dT) cellulose washing, mRNA elution, and precipitation steps.

What's inside

• Lysate Preparation
• Oligo(dT) Cellulose Washing
• mRNA Elution
• Precipitation
• Storage

Frequently asked questions

What lysate volumes are required for the initial incubation step?

The protocol requires incubating a 15 ml lysate at 45°C for 15 to 60 minutes.

What are the centrifugation settings for the initial lysate processing?

Centrifuge the sample at 4,000 x g for 5 minutes at room temperature.

How much sodium acetate and ethanol are added to precipitate the mRNA?

Add 60 µl of 2 M sodium acetate and 1 ml of 100% ethanol to the eluent.

What are the final storage conditions for the isolated mRNA?

Store the mRNA at -70°C or use it immediately.