This manual describes how to use the ViraPower™ Lentiviral Expression Systems. These systems are for high-level expression in dividing and non-dividing mammalian cells.
The document serves as a user guide for the ViraPower™ Lentiviral Expression Systems, which are designed for high-level gene expression in both dividing and non-dividing mammalian cells. The manual covers various kit types, including Directional TOPO® and Gateway® expression kits, as well as specific support kits like Bsd and Zeo variants.
Key sections include detailed information on kit contents, proper storage conditions for components such as the 293FT cell line and Lipofectamine™ 2000, biosafety features, experimental outlines, and step-by-step methods for producing lentivirus in 293FT cells. The guide also addresses titering viral stocks, transduction analysis, troubleshooting common issues, and provides technical support contact information.
| Target Cell Types | dividing and non-dividing mammalian cells |
|---|---|
| Producer Cell Line | 293FT |
| Packaging Mix Storage Temperature | -20°C |
| Lipofectamine 2000 Storage Temperature | 4°C (do not freeze) |
| Blasticidin Storage Temperature | -20°C |
| Zeocin Storage Conditions | -20°C, protected from light |
| 293FT Cell Line Storage Condition | liquid nitrogen |
The system uses 293FT producer cells for producing the lentivirus.
Yes, the ViraPower™ Lentiviral Expression Systems are designed for high-level expression in both dividing and non-dividing mammalian cells.
It must be stored at 4°C, and it is critical not to freeze the reagent.