Invitrogen pET-DEST42 Gateway Vector User Manual

Provides product information for the pET-DEST42 Gateway™ Vector. This vector is designed for high-level, inducible expression in E. coli


Invitrogen pET-DEST42 Gateway Vector User Manual - cover page
Brand
invitrogen
Document type
User Manual
Language
English
Pages
24
File format
PDF
File size
487 KB
Published
16 October, 2023
Updated
16 October, 2023
MD5 checksum
4C7011DDAEE7B9435F7FA3CD1A762A40

About this manual

The Invitrogen pET-DEST42 Gateway Vector is a 7.4 kb destination vector designed for use with Gateway Technology to allow high-level, inducible expression of recombinant fusion proteins in E. coli using the pET system. It contains a T7lac promoter for IPTG-inducible expression and includes elements such as V5 epitope and 6xHis tag sequences for detection and purification.

What's inside

  • Important Information
  • Accessory Products
  • Overview
  • Using pET-DEST42
  • BL21 E. coli Strains
  • Expression and Analysis
  • Map and Features of pET-DEST42

Specifications

Vector Size7.4 kb
Resistance GeneAmpicillin resistance gene
Origin of ReplicationpBR322 origin
Epitope TagV5 epitope and 6xHis tag

Frequently asked questions

What is the recommended storage temperature for pET-DEST42?

Upon receipt, store at -20 °C.

How can a true expression clone be confirmed?

True expression clones will grow on ampicillin but not on chloramphenicol.

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