This manual provides information on the use of pBAD/His and pBAD/Myc-His vectors, which are versatile tools for the expression of recombinant proteins in E. coli.
This manual provides instructions for using the pBAD/His and pBAD/Myc-His vector kits (Catalog nos. V430-01 and V440-01) for the regulated, dose-dependent expression and purification of recombinant proteins in E. coli. The vectors utilize the araBAD promoter (PBAD) regulated by the AraC protein and L-arabinose to control protein expression levels.
| Vector Sizes | pBAD/His A (4102bp), pBAD/His B (4092 bp), pBAD/His C (4100 bp) |
|---|---|
| Selection Marker | Ampicillin resistance |
| Origin of Replication | pBR322 origin |
| Regulatory Protein | AraC |
They are designed for regulated, dose-dependent recombinant protein expression and purification in E. coli.
Expression is regulated by the araBAD promoter (PBAD) using the regulatory protein AraC and the inducer L-arabinose.