Invitrogen K450-01 pLEX Expression System User Manual

About this manual

The document details the Invitrogen PLEX Expression System (Catalog No. K450-01), a prokaryotic system designed for the expression of heterologous proteins in E. coli. The system utilizes a tryptophan-inducible expression mechanism driven by the strong PL promoter from bacteriophage lambda, allowing for high-level production even of potentially toxic proteins.

What's inside

• Overview
• Cloning into PLEX
• Transformation
• Expression
• Important Information
• Recipes
• Technical Service

Frequently asked questions

What is the catalog number of this prokaryotic expression system?

The product is identified by Catalog no. K450-01.

At what temperature should lyophilized vectors and primers be stored?

Lyophilized vectors and primers must be stored at -20°C.

What is the genotype of the G1724 E. coli strain included in the kit?

The G1724 strain has the genotype FX, lacl4, lacPL8, ampC::P,,, el, merA, merB, INV@nnD-rmnE.