This technical note describes the C-Terminal 5.0 protein sequencer methods.
This document describes the C-terminal 5.0 sequencer methods which implement thiohydantoin degradation chemistry on the Hewlett-Packard column-based protein sequencer. The methods are suitable for C-terminal sequence analysis of most protein samples in the low nanomole to 50 pmole range applied onto a Hewlett-Packard Zitex strip.
| Sample Range | low nanomole to 50 pmole |
|---|---|
| Column Type | empty RP/SAX biphasic column |
| Strip Type | Hewlett-Packard Zitex strip |
| Total Time (C-Terminal 5.0) | 75.2 min |
| Total Time (C-Terminal 5.0 Cycle 1) | 88.7 min |
They implement the thiohydantoin degradation chemistry (coupling and cleavage).
No, reagents R1, R2A, R4, and the PTH-Std are specific for N-terminal sequencing and are not utilized.