Hewlett-Packard 241 N-Terminal 5.0 Biphasic Column Sequencer Methods Manual

This technical note introduces how to use the Hewlett-Packard 241 Protein Sequencer (N+C) N-Terminal 5.0 Biphasic Column Sequencer Methods to analyze proteins and peptides, including the principle, operating steps, precautions, etc.

Brand: HP
Category: Other
Document type: User Manual
Language: English
Pages: 9
File format: PDF
File size: 104 KB
Published: 05 July, 2012
Updated: 30 September, 2023
MD5 checksum: EAB5A015407C4EAE4273CC09EA865E76
PDF: Hewlett-Packard 241 N-Terminal 5.0 Biphasic Column Sequencer Methods Manual PDF

About this manual

This document provides technical notes and methods for the N-terminal 5.0 biphasic column sequencer, designed for use with the Hewlett-Packard 241 Protein Sequencer (N+C). The methods implement Edman degradation chemistry for analyzing proteins and peptides in the low nanomole to low picomole range.

What's inside

N-Terminal Protein Column 5.0
N-Terminal Peptide Column 5.0
N-Terminal Flask 5.0
PTH-Std 5.0
Column Prep 5.0
Reagent Configuration
Method Details
Cycle 1 Method
Wash and Dry Steps
Extraction and Cleavage

Specifications

Sample Range	low nanomole to low picomole
Standard Delivery	10 pmol/100 pl
Column Configuration	reverse-phase (RP) sample column mated with a strong anion exchange (SAX) column

Frequently asked questions

What is the purpose of the Column Prep 5.0 method?

It is used to wash the sequencer column prior to sample loading, which is particularly important for low-level sample quantities.

What is the difference between column methods for peptides and proteins?

For proteins, 52A is used as an extraction and wash solvent, while 2/3 and 3 are used for peptides to lessen wash-out.

What does the Cycle 1 method provide for the N-terminal protein column?

It provides a longer initial drying time and a pause for proper HPLC column equilibration after switching from C-terminal sequencing.

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