N-to-C 5.0 and C-to-N 5.0 sequencer methods are responsible for switching between the N-terminal and C-terminal sequencing chemistry methods on the Hewlett-Packard column-based protein sequencer
This document provides technical details on the N-to-C 5.0 and C-to-N 5.0 methods used by the Hewlett-Packard column-based protein sequencer to switch between sequencing chemistries. It explains that these methods manage the transition from N-terminal to C-terminal or vice versa, utilizing either a column method or a flask method depending on the specific requirement.
| Total Time (Column Wash) | 45.5 min |
|---|---|
| Total Time (N-to-C Column Method) | 20.7 min |
| Total Time (Flask Wash) | 67.1 min |
| Total Time (N-to-C Flask Method) | 11.2 min |
These methods are responsible for switching between the N-terminal and C-terminal sequencing chemistry methods on the sequencer.
The N-to-C 5.0 column method requires a total time of 20.7 minutes.
After Step 3, approximately 70 µl (+/- 5 µl) of R4 should be delivered to the flask.
The N-to-C 5.0 flask method has a total time of 11.2 minutes.