This note discusses the effect of these collision parameters in triple-quadrupole and ion-trap mass spectrometers. It shows how the new SmartFrag feature in the Agilent 1100 Series LC/MSD Trap ion-trap mass spectrometer overcomes compound dependency to generate optimum information from every analyte.
The document discusses the challenges of obtaining consistent mass spectrometry results, specifically regarding compound-dependent collision optimization. It contrasts triple-quadrupole instruments with traditional ion traps, noting that fixed settings often yield poor structural information for certain compounds.
A new feature called SmartFrag is introduced to address this issue. By utilizing CID voltage ramping within the Agilent 1100 Series LC/MSD Trap, the instrument automatically varies collision energy over a range of values. This ensures optimum fragmentation and richer product ion spectra without requiring manual optimization for each specific analyte.
SmartFrag is a new feature built into the firmware of the Agilent 1100 Series LC/MSD Trap that uses CID voltage ramping to eliminate the need for optimizing collision energy for different compounds.
It quickly ramps the collision voltage over a range of energies each time MS/MS is performed, ensuring that each compound receives the correct energy for optimum fragmentation.