This Technical Overview evaluates the performance of the new 80-nL cell designed for micro bore and capillary LC UV detection. Using a standard chromatographic separation allows to compare analytical parameters of the 500-nL cell to those of the new 80-nL cell. This clearly demonstrates a great improvement in chromatographic separation.
This document presents a technical evaluation of the new Agilent 80-nL flow cell designed specifically for micro bore and capillary liquid chromatography (LC) with UV detection. The study compares the analytical parameters of this new cell against the previous 500-nL model using standard chromatographic separations.
It is designed for micro bore and capillary LC UV detection.
Detection was performed at 254 nm with a reference wavelength of 360 nm.
A flow rate of 4 µL/min was used for the 0.3 mm column.