Agilent 2100 bioanalyzer's Small RNA assay method, which can detect size from 6-150nt small RNA
The document details the capabilities of the Agilent Small RNA assay, which analyzes small nucleic acids ranging from 6 to 150 nucleotides. It is designed to run on the Agilent 2100 bioanalyzer and can visualize miRNA, small RNA, and oligonucleotides while verifying sample integrity.
The note covers the linear dynamic range of quantification for miRNA (50 - 2000 pg/pL) and provides recommended concentrations for different sample types. It also outlines analytical specifications such as analysis run time, sensitivity, and reproducibility.
| Analysis range | 6-150 nt |
|---|---|
| Quantitation reproducibility | 25 % CV (for ladder as an example) |
| Analysis run time | 30 minutes |
| Number of samples per chip | 11 samples/chip |
| Assay kit stability | 4 months |
The assay can quantify miRNA in the concentration range of 50 - 2000 pg/pL relative to an external standard.
The recommended concentration for Total RNA samples is between 1-100 ng/pL.
An analysis run takes approximately 30 minutes.