This document introduces the design method of 1100 LC/MSD system, including cool non-heated capillary, HPLC flow rate conditions set independently of the ion optics lens in the high vacuum region of the mass spectrometer.
This document explains how to use the Flow Injection Analysis (FIA) feature in the Agilent 1100 Series LC/MSD software to optimize mass spectrometry parameters. Unlike traditional infusion pump methods, FIA allows for faster, automated, and repeatable optimization of source conditions without requiring a column or system re-plumbing. The process involves setting up a series of injections with varying parameters to identify the optimum settings for sensitivity.
| Time between Injections | 0.8 min |
|---|---|
| Fragmentor voltage range | 30-150 volts |
| APCI vaporizer temperature range | 300-500°C |
| Capillary voltage range | 2500-5000 volts |
| APCI corona current range | 2-10 microamps |
| Drying gas temperature range | 150-350°C |
| Drying gas flow rate range | 3-13 L/min |
| Nebulizing gas pressure range | 10-60 psig |
The time between injections is typically set at 0.8 minutes for applications without a column.
FIA optimization is much faster than infusion because it provides automation of the optimization steps.
The recommended step for fragmentor voltage optimization is 10 volts.