This document introduces the features and applications of Agilent active splitter and delay sensor
This document is a technical note detailing the optimization of fraction collection using Agilent LC/MSD systems equipped with an active splitter and a delay sensor. It highlights the benefits of these components for improving productivity in early drug discovery, such as collecting pure samples and minimizing analysis time. The text explains the operational features of the active splitter, including its ability to vary split ratios and provide early maintenance feedback (EMF). Additionally, it describes the delay sensor's role in accurately calculating time delays between peak detection and fraction collection, ensuring valuable fractions are not lost.
The calibration uses a sample containing a dye and caffeine injected together with no chromatographic column.
The seal replacement kit requires only about five minutes to install.
The caffeine ion at m/z 195 is monitored by the LC/MSD during the calibration process.