Varian Evaporative Light Scattering Detector is a technology that enhances LC-MS and can detect compounds that have little or no UV chromophore.
The Varian Evaporative Light Scattering Detector (ELSD) functions independently of the optical properties of the analyte, making it ideal for detecting compounds that are difficult to analyze with standard UV detection. It is designed to be coupled with LC-MS systems, often with a flow split, to provide concentration information that complements the structural data from mass spectrometry.
The document illustrates the benefits of using the Varian ELSD in pharmaceutical analysis, specifically in high throughput screening (HTS) applications. It demonstrates how the ELSD can accurately determine the ratio of compounds like verapamil and diazepam in a mixture, correcting discrepancies found when relying solely on LC-MS or UV detection.
The detector responds to all compounds that are less volatile than the mobile phase.
The UV results were calculated at a wavelength of 254 nm.
The ratio of verapamil to diazepam is calculated at 3:1 by LC-MS.