This document introduces the advantages of Varian OneNMR™ probe, including excellent lineshape performance, superior B1 field homogeneity and outstanding salt tolerance. The probe uses a solvent suppression technique called “Presaturation Utilizing Relaxation Gradients”, or PURGE, which has very efficient and robust solvent suppression effects, excellent frequency selectivity, and no baseline perturbations outside of the suppression window.
The Varian OneNMR Probe is designed to deliver high-quality data for demanding metabolomic analysis by combining excellent lineshape performance, superior B1 field homogeneity, and outstanding salt tolerance in a single probehead. Historically, no single design could meet all these specifications simultaneously without compromise.
Key features include the implementation of the PURGE (Presaturation Utilizing Relaxation Gradients) technique for efficient solvent suppression with high frequency selectivity. The probe also offers outstanding deuterium channel sensitivity, allowing automated locking and shimming on samples containing as little as 2% D2O. Additionally, its unique design minimizes signal loss in highly dielectric samples, providing superior salt tolerance compared to classic inverse detection probes.
PURGE stands for Presaturation Utilizing Relaxation Gradients, a solvent suppression technique involving hard rotations interspersed with short periods of gentle presaturation excitation.
The OneNMR Probe allows automated locking and shimming on samples containing as little as 2% D2O.