Agilent PLRP-S media are sufficiently robust and mechanically stable to pack into efficient microbore columns with internal diameters of 1.0 mm and 2.1 mm. PLRP-S microbore columns offer substantially increased sensitivity over standard bore columns, mainly due to the solvent content being much reduced, at comparative linear velocities. The improvement in sensitivity that can be gained by using microbore columns compared to traditional 4.6 mm analytical columns is clearly evident.
This document provides a technical overview of Agilent PLRP-S media packed into efficient microbore columns with internal diameters of 1.0 mm and 2.1 mm. These columns offer substantially increased sensitivity over standard bore columns, primarily due to reduced solvent content at comparative linear velocities.
The text details the benefits of using microbore systems, including lower chemical and disposal costs, reduced sample losses, and improved peak shape. It presents data comparing the performance of 1.0 mm, 2.1 mm, and standard 4.6 mm columns for various applications, such as analgesic separation (aspirin, phenacetin, salicylic acid) and peptide analysis (oxytocin, angiotensins, insulin), highlighting significant improvements in detection limits.
Agilent PLRP-S media can be packed into efficient microbore columns with internal diameters of 1.0 mm and 2.1 mm.
The detection limit was 25.33 pg for the 1.0 mm id column.
The lower detection limit of the 1 mm id column was 0.975 ng.