Agilent ZORBAX SB-C8 RRHT threaded columns are specially designed for higher pressure operation and are packed with a high-performance microparticulate C8 packing for high-speed reversed-phase HPLC. The StableBond packing is made by chemically-bonding a sterically-protected C8 stationary phase to a specially-prepared, high-purity ZORBAX porous silica microsphere. The special ZORBAX silica support is designed to reduce or eliminate strong adsorption of basic compounds. The densely-covered, sterically-protected, diisopropyl n-octylsilane stationary phase is chemically stable and gives longer column life. It is particularly well-suited for use with aggressive mobile phases (for example, pH <2, high ionic strength, ion- pair additives, etc.) since the steric protection of the bonded phase resists degradation caused by such mobile phases. This material has also been shown to be stable when operated at temperatures up to 80 °C. These characteristics are particularly important for use in methods that need long-term stability and reproducibility. ZORBAX SB-C8 is especially suited in applications that use high-sensitivity detectors that require low backgrounds (for example, mass spectrometers). The uniform, spherical, ZORBAX SB-C8 particles have a controlled pore size of 80Å. The columns are 250 mm long, 4.6 mm inner diameter, and the particle size is 5 μm.
This document describes the Agilent ZORBAX SB-C8 RRHT threaded columns, which are engineered for high-pressure liquid chromatography (HPLC) operations up to 600 bar. The columns utilize StableBond technology, featuring a sterically-protected C8 stationary phase chemically bonded to high-purity ZORBAX porous silica microspheres. This design reduces the adsorption of basic compounds and provides enhanced stability against aggressive mobile phases, including those with low pH or high ionic strength, as well as elevated temperatures up to 80 °C.
| Maximum operating pressure | 600 bar (9000 psi) |
|---|---|
| Maximum operating temperature | 80 °C |
| Particle size | 1.8 µm |
| Pore size | 80 Å |
| Compatible pH range | pH 1.0 to 8.0 |
The maximum operating pressure is 600 bar (9000 psi).
Yes, the columns are stable when operated at temperatures up to 80 °C.
The columns use a sterically-protected diisopropyl n-octylsilane stationary phase.